Tuesday 17th of September
17:00 - 20:00 Registration and Welcome Reception - Aula Vetmeduni Vienna
Program: 17th Avian Immunology Research Group Meeting lecture hall B
8.00 Registration
9.00 Welcome and opening Dieter Liebhart and Michael Hess
9.15 - 10.15 Keynote I - Chair: Tina Sorensen Dalgaard
Cross-species conservation of host factors and virus evolution highlight proteins at the antiviral frontline
Andreas Pichlmair1
Institute of Virology, Technical University of Munich, Germany
10.15 - 11.30 Session I (Host-Pathogen Interactions I) - Chair: Tina Sorensen Dalgaard
11.30 - 12.00 Coffee Break and Poster Session
12.00 - 13.00 Session II (Host-Pathogen Interactions II) - Chair: Christine Jansen
Impact of microbial populations on the intestinal immune system14.00 - 15.00 Session III (Host-Pathogen Interactions III) - Chair: Thomas Göbel
Changes in cellular immune response in lung and spleen coincidence with pathological lesions and organ colonization of broilers and layer type chickens foll9owing experimental infection with avian pathogenic Escherichia coliEffects of prior pathogen exposure on inter-individual variability in immunity and disease in house finches
Garrett-Larsen, J. N.1*, Pérez-Umphrey1*, A. A, Langwig1, K. E., Fleming-Davies2, A. E. & D. M. Hawley11Department of Biological Sciences, Virginia Tech, Blacksburg, VA, USA; 2Department of Biology, University of San Diego, San Diego, CA, USA *These authors contributed equally.
Regulation of the house finch population-specific immune responses to an evolving pathogen, Mycoplasma gallisepticum
Michal Vinkler1*, Amberleigh E. Henschen2, Nithya Kuttiyarthu Veetil1, Balraj Melepat1, Daniel Divín1, Dana M. Hawley3, Rami A. Dalloul4, James S. Adelman2*
1Charles University, Faculty of Science, Department of Zoology, Prague, Czech Republic; 2University of Memphis, Department of Biological Sciences, Memphis, USA; 3Virginia Tech, Department of Biological Sciences, Blacksburg, USA; 4University of Georgia, Department of Poultry Science, Athens, USA *These authors contributed equally.
Effects of early-life immune activation on behaviour in budgerigars (Melopsittacus undulatus)
Marková K.1, Divín D.1, Voukali E.1, Melepat B.1, Těšický M.1, Veetil N. K.1, Li T.1, Szabó A.1, Exnerová A., Vinkler M.1
1Department of Zoology, Faculty of Science, Charles University, Czech Republic
Different immune genes show variation in strength of positive selection: genomic approach in birds
Martin Těšický1,2,3, Wieslaw Babik4, Jakub Kreisinger1, Seran Yıldız1, Laurent Frantz3, Tomáš Albrecht1,2, Michal Vinkler1
1Charles University, Faculty of Science, Department of Zoology, Prague, Czech Republic; 2Institute of Vertebrate Biology, v.v.i., The Czech Academy of Sciences, Brno, Czech Republic; 3Ludwig Maximilian University of Munich, Faculty of Veterinary Medicine, Institute of Palaeoanatomy, Domestication Research and the History of Veterinary Medicine, Munich, Germany; 4Institute of Environmental Sciences, Faculty of Biology, Jagiellonian University, Krakow, Poland
Omission of dietary P supplements affects immune cells numbers but not function in laying hens
N. Wallauch1, S. Schmucker1, T. Hofmann1, V. Sommerfeld1, K. Huber1, M. Rodehutscord1, V. Stefanski1
1Institute of Animal Science, University of Hohenheim, 70599 Stuttgart, Germany
14.00 - 15.15 Session VI (Vaccines and Immungenetics) - Chair: Michael Hess
Cell mediated responses generated in chicken immunised with H9N2 avian influenza irradiated vaccine
Richard Thiga Kangethe1, Alessio Bortolami2, Francesco Bonfante2, Giovanni Cattoli2, Viskam Wijewardana1
1Animal Production and Health Laboratory, Department of Nuclear Sciences and Applications, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency (IAEA), Vienna, Austria; 2Department of Comparative Biomedical Sciences, Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro, Italy
The French touch: feedback, challenges and prospects of vaccination against high pathogenicity influenza viruses in duck farming
Sébastien Mathieu Soubies1, Mathilda Walch1, Clément Castille1, Laura Lebouteiller1, Guillaume Croville1, Jean-Luc Guérin1
1IHAP, Université de Toulouse, INRAE, ENVT, Toulouse, France
Spatio-temporal distribution of HVT and innate immune responses following in ovo inoculation
Emma Armstrong1, Kate Sutton1, Ad de Groof2, Jayne Hope1, Lonneke Vervelde1
1The Roslin Institute, The University of Edinburgh, Easter Bush Campus, Midlothian, United Kingdom; 2MSD-Animal Health, Boxmeer, Netherlands
Efficacy and tolerability of an mRNA vaccine expressing gB and pp38 antigens of Marek’s disease virus in chickens
Fatemeh (Darya) Fazel1, Nitish Boodhoo1, Ayumi Matsuyama-Kato1, Mohammadali Alizadeh1, Shayan Sharif1
1Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada
15.15 - 16.00 Coffee Break and Poster Session
19.00 Conference Dinner in Vienna
9.00 - 10.00 Keynote III - Chair: Gisela Erf
How much diversity is in your breakfast egg? Local breed or high-performance chickens: who is more immunologically competent?
Steffen Weigend1 and Ulrike Blohm2
1Friedrich Loeffler Institut, Institute of Farm Animal Genetics, Neustadt-Mariensee, Germany; 2Friedrich Loeffler Institut, Institute of Immunology, Greifswald – Insel Riems, Germany
10.00 - 10.30 Session VII (Vaccines and Immungenetics) - Chair: Gisela Erf
Optimising in ovo Herpesvirus of turkey (HVT) - vectored vaccines: Defining the role of the HVT vNr-13 protein in vitro in chicken embryo fibroblasts and in ovo in late stage embryonic tissues
Vishwanatha R. A. P. Reddy1, Weicheng Li2, Yaoyao Zhang2, Salik Nazki3, 4, Andrew J. Broadbent2, 5, Yongxiu Yao2, Venugopal Nair2, 6, 7
School of Life Sciences, Keele University, Keele, United Kingdom; 2The Pirbright Institute, Woking, United Kingdom; 3Pandemic Sciences Institute, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom; 4Chinese Academy of Medical Sciences Oxford Institute, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom; 5Department of Animal and Avian Sciences, University of Maryland, United States of America; 6Jenner Institute, University of Oxford, Oxford, United Kingdom; 7Department of Biology, University of Oxford, Oxford, United Kingdom
Characterizing expression profiles of turkey immune cell populations within peripheral blood using single-cell transcriptomics
Melissa S. Monson1, Sharu Paul Sharma2, Kristen A. Byrne1, and Crystal L. Loving1
1USDA, ARS, National Animal Disease Center, Food Safety and Enteric Pathogens Research Unit, Ames, IA, USA; 2Iowa State University, Office of Biotechnology, Genome Informatics Facility, Ames, IA, USA
10.30 - 11.00 Coffee Break and Poster Session
11.00 - 12.00 Session VIII (Innate Immunity) - Chair: Michal Vinkler
Aberrant innate immune function in UCD200/206 scleroderma-prone chickens
Gisela F. Erf1, Chrysta N. Beck1, Jossie M. Santamaria1, Angeline Rodriguez1, Katee Johnson1, Allie Bowerman1
1University of Arkansas System Division of Agriculture, Department of Poultry Science, Fayetteville, Arkansas, USA
Developing cell based assays to study trained immunity in chicken NK cells
Daphne van Haarlem1, Claudia van Hal1, Christine Jansen1
1Cell Biology& Immunology group, Department of Animal Sciences, Wageningen University & Research, Wageningen, The Netherlands
Characterization of nucleotide-binding oligomerization domain (NOD)-like receptor protein 3 (NLRP3) in chickens and its role in Marek’s disease
Janan Shoja Doost1, Nitish Boodhoo1, Shayan Sharif1
1Department of Pathobiology, University of Guelph, Ontario, Canada
Avian interferon regulatory factors (almost) resolved
Lenka Ungrová1, Jiří Nehyba1,Veronika Krchlíková2, Tomáš Hron1, Bernd Kaspers3, Josef Geryk1, Jiří Hejnar1, Daniel Elleder1
1Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic; 2Institute for Medical Virology and Epidemiology of Viral Diseases, Tuebingen, German; 3 Department of Veterinary Science, Faculty of Veterinary Medicine at the Ludwig-Maximilians-University, Planegg/Martinsried, Germany
12.00 - 12.30 Coffee Break and Poster Session
12.30 Presentation of the Poster Award, Business Meeting and conference closing
POSTER: 17th Avian Immunology Research Group Meeting
Dietary fiber supplementation and Campylobacter jejuni effects the cellular immune response in the cecum of broiler chickens
Sina Bagheri1, Motuma Debelo1, Dieter Liebhart1, Claudia Hess1, Michael Hess1, Wageha Awad1
1Clinical Centre for Population Medicine in Fish, Pig and Poultry, Clinical Department for Farm Animals and Food System Science, University of Veterinary Medicine, Vienna, Austria
Absolute cell count highlights the importance of CD4+ and CD8+ T cells against hepatitis-hydropericardium syndrome (HHS) challenge after vaccination with a fowl adenovirus (FAdV) chimeric fiber protein
Carlotta De Luca1,2, Anna Schachner2, Michael Hess1,2, Dieter Liebhart1, Taniya Mitra1
1Clinical Centre for Population Medicine in Fish, Pig and Poultry, Clinical Department for Farm Animals and Food System Science, University of Veterinary Medicine, Vienna, Austria; 2Christian Doppler Laboratory for Innovative Poultry Vaccines (IPOV), University of Veterinary Medicine, Vienna, Austria
Characterization of a novel chicken γδ TCR specific marker
Veronika Drexel1, Simon Früh1, 2, Thomas Göbel1
1Department of Veterinary Immunology, LMU Munich, Germany; 2Department of Veterinary Medicine, Institute of Virology, FU Berlin, Germany
In vitro immunoregulatory potential of A.galli excretory/secretory products
Feix, AS1, Kjærup, RB1, Premathilaka, C2, Kodithuwakku, S2, Fazeli, A2, Dalgaard, TS1
1Department of Animal and Veterinary Sciences, Aarhus University, Tjele, Denmark; 2 ERA Chair COMBIVET, Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Science, Tartu, Estonia
Comparative immune competence analysis of three local chicken breeds
Luise Freier1, Inga Tiemann2, Josefine Stuff2, Steffen Weigend3, Maryna Kuryshko4, Elsayed M. Abdelwhab4, Christian Grund5, Ulrike Blohm1
1Friedrich Loeffler Institut, Institute of Immunology, Greifswald – Insel Riems, Germany; 2Institute of Agricultural Engineering, University of Bonn, Germany; 3Friedrich Loeffler Institut, Institute of Farm Animal Genetics, Neustadt-Mariensee, Germany; 4Friedrich Loeffler Institut, Institute of Molecular Virology and Cell Biology, Greifswald – Insel Riems, Germany; 5Friedrich Loeffler Institut, Institute of Diagnostic Virology, Greifswald – Insel Riems, Germany
From B cells to T cells – identification of IBDV specific T helper cells by using immunopeptidomics
Samer Halabi1, Michael Ghosh2, Maria Danysz1, Stefan Stevanović2, Hans-Georg Rammensee2, Jim Kaufman1, Sonja Härtle3
1Institute for Immunology and Infection Research, University of Edinburgh, Edinburgh, United Kingdom; 2Department of Immunology, Institute of Cell Biology, University of Tübingen, Tübingen, Germany; 3Department of Veterinary Sciences, LMU Munich, Planegg, Germany
Inhibition of Marek’s disease virus replication and spread by 25-hydroxycholesterol and 27-hydroxycholesterol in vitro
Nitin Kamble1, Vishwanatha R. A. P. Reddy1,2, Ben Jackson1, Faisal R. Anjum1,3, Chidiebere C. Ubachukwu1, Ajit Patil1, Shahriar Behboudi1,3
1The Pirbright Institute, Ash Road, Woking, United Kingdom; 2School of Life Sciences, Keele University, Keele, United Kingdom; 3Bristol Veterinary School, University of Bristol, Bristol, United Kingdom
Systemic immunomodulatory effects of phytogenic feed additives in Ascaridia galli infected layers
Kjærup, RB1, Feix, A1, Thamsborg, SM2, Rotovnik, R2, Mejer, H2, Permin, A3, Dalgaard, TS1
1Deptartment of Animal and Veterinary Sciences, Aarhus University, Tjele, Denmark; 2Deptartment of Veterinary and Animal Sciences, University of Copenhagen, Denmark; 3Værløse Dyreklinik IVS, Denmark and Unibrains IVS, Denmark
Immunomodulatory effects of Bacillus subtilis DSM 32315 and Bacillus velezensis CECT 5940 on chicken peripheral blood mononuclear cells
F. Larsberg1,2, M. Sprechert2, D. Hesse2, G. Loh3, G.A. Brockmann2, S. Kreuzer-Redmer1
1Vetmeduni Vienna, Centre for Animal Nutrition and Animal Welfare Sciences, Vienna Austria; 2Humboldt-Universität zu Berlin, Albrecht Daniel Thaer-Institute, Breeding Biology and Molecular Genetics, Berlin Germany; 3Evonik Operations GmbH – Research, Development & Innovation Nutrition & Care, Hamburg, Germany
Towards flow cytometric profiling of zebra finch leukocytes
Balraj Melepat1, Karsten Skjødt2, Tina Sørensen Dalgaard3, Michal Vinkler1
1Charles University, Faculty of Science, Department of Zoology, Prague, Czech Republic; 2University of Southern Denmark, Department of Molecular Medicine, Odense, Denmark; 3Aarhus University, Department of Animal and Veterinary Sciences, Blichers Allé, Denmark
Escherichia coli vaccination and chitin feed supplementation as alternative methods to control Ascaridia galli infection in chickens
Moosavi, M1,2, Kjærup, RB1, Wattrang, E3, Dalgaard, TS1
1Department of Animal and Veterinary Sciences, Aarhus University, Tjele, Denmark; 2Department of Poultry Science at Tarbiat Modares University, Tehran, Iran; 3Department of Microbiology, Swedish Veterinary Agency, Uppsala, Sweden
Characterization of regulatory T cells in chickens
Isabell Naumann1, Bernd Kaspers1, Simon Früh1,2, Thomas Göbel1, Sonja Härtle1
1Department for Veterinary Sciences, Ludwig-Maximilians-Universität Munich, Munich, Germany; 2Department of Veterinary Medicine, Institute of Virology, Freie Universität Berlin, Berlin, Germany
A class II system strongly expressed in intestinal epithelial cells after helminth worm infection
Aimee Parker1, Tina Dalgaard2 Jim Kaufman3
1Quadram Institute, Norfolk, United Kingdom; 2Aarhus University, Aarhus, Denmark; 3University of Edinburgh, Edinburgh, United Kingdom
In vitro assessment of immunogenicity and metabolic activity of different gamma-irradiated Escherichia coli strains
Hammad Ur Rehman1, Sina Bagheri1, Mohamed Kamal Abdelhamid1, Surya Paudel2, Viskam Wijewardana3 Claudia Hess1, Michael Hess1, Dieter Liebhart1
1Clinical Centre for Population Medicine in Fish, Pig, and Poultry, Clinical Department for Farm Animals and Food System Science, University of Veterinary Medicine Vienna, Austria; 2Department of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong; 3Animal Production and Health Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency IAEA, Vienna, Austria
Antigenic cartography analysis of chimeric recombinant IBDV strains can be used to identify hypervariable region mutations that induce broadly cross-neutralizing antibody responses against diverse strains
Vishwanatha RAP Reddy1,2, Sofia Egana-Labrin3, Salik Nazki1,4, Andrew Broadbent1,3
1The Pirbright Institute, Ash Road, Woking, UK; 2School of Life Sciences, Keele University, Keele, ST5 5BG, UK; 3Department of Animal and Avian Sciences, University of Maryland, 8127 Regents Drive, College Park, Maryland, 20742, USA; 4Institute of Developmental and Regenerative Medicine, IMS Tetsuya Nakamura Building, Old Road Campus, University of Oxford, Oxford, UK
Suppressive effects of cyclic, environmental heat stress on the local and systemic acute inflammatory responses to lipopolysaccharide and on the circulating levels of lymphocytes in broiler chickens
Alessandro J. Rocchi1, Jossie M. Santamaria1, Chrysta N. Beck1, Gisela F. Erf1
1University of Arkansas System Division of Agriculture, Department of Poultry Science, Fayetteville, Arkansas, USA
Development of an intestinal inflammation model in poultry to apply new solutions modulating innate immunity
J. Schmeisser1, R. Aureli1, C. Chatelle1, P. Jenn1, C. Iaconis1, E. Bacou1, A. Leduc1
1dsm-firmenich, Animal Nutrition and Health R&D Center, Village Neuf, France
Tenascin-C regulates B-cell migration in developing avian bursa of Fabricius
Ádám Soós1, Emőke Szőcs1, Viktória Halasy1, Nándor Nagy1
1Semmelweis University, Budapest, Hungary
Conventional dendritic cells limit infectious bronchitis virus in chicken lung
Samantha Sives1, Emma Armstrong1, Dominika Borowska1, Zhiuguang Wu1, Adam Balic2,3, Kate Sutton1
1Division of Immunology, The Roslin Institute and R(D)SVS, University of Edinburgh, UK: 2The Roslin Institute and Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Midlothian, UK; 3Department of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, Victoria, Australia
Characterization and ontogeny of a novel lymphoid follicle inducer cell during development of the bursa of Fabricius
Emőke Szőcs1, Adam Balic2,3, Ádám Soós1, Viktória Halasy1, Nandor Nagy1
1Department of Anatomy, Histology and Embryology, Faculty of Medicine, Semmelweis University, Budapest, Hungary; 2The Roslin Institute and Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Midlothian, UK; 3Department of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, Victoria, Australia
Immune response of γδ T cell knockout chickens after Salmonella enterica serovar Enteritidis infection
Felix Tetzlaff1, Ulrich Methner2, Benjamin Schusser3, Christian Menge1, Angela Berndt1
1Institute of Molecular Pathogenesis, Friedrich-Loeffler-Institute, Jena, Germany; 2Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institute, Jena, Germany; 3Reproductive Biotechnology, Department of Molecular Life Sciences, School of Life Sciences Weihenstephan, Technical University Munich, Freising, Germany
The knockout of αβ T cells causes severe cytotoxic reactions in chicken – new insights into the functions of T cell subpopulations
Theresa von Heyl1, Romina Klinger1, Dorothea Aumann1, Christian Zenner1, Mohanned Alhussien1, Antonina Schlickenrieder1, Kamila Lengyel1, Hanna-Kaisa Vikkula1, Teresa Mittermair1, Hicham Sid1, Benjamin Schusser1,2*
1Reproductive Biotechnology, TUM School of Life Sciences, Weihenstephan, Freising, Germany; 2Center for Infection Prevention (ZIP), Technical University of Munich, Freising, Germany
Detection and enumeration of Eimeria tenella-specific IFN-γ producing spleen cells and PBMC by ELISpot
Eva Wattrang1, Osama Ibrahim1, Anna Lundén1
1Department of Microbiology, Swedish Veterinary Agency, Uppsala, Sweden
Immune responses of chickens against recombinant Salmonella enterica serotype Infantis flagellar and fimbrial proteins
Hung-Yueh Yeh1, Jonathan G. Frye1, Charlene R. Jackson1
1U.S. Department of Agriculture, Agricultural Research Service, U.S. National Poultry Research Center, Athens, USA
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